Life Inside a Thin Section: Tomography
نویسندگان
چکیده
منابع مشابه
Life inside a thin section: tomography.
Introduction Electron microscopic (EM) tomography is proving useful for examining the organization of protein molecules in large molecular machines at critical neural sites, such as synapses. Methods are still in a state of flux, and many steps exist between a neuron and a molecular model of one of its synapses. Here, we show our approach for doing tomography on synaptic structure , hoping ther...
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Thin section polarized-light microscopy is a simple, cost-effective and nondestructive technique utilized to delineate crystalline or amorphous solids at varying scales and spatial resolutions; thereby allowing the different phases to be studied within their textural framework. Estimates of chemical composition and insight into the solid’s genesis can be deduced via unique textural characterist...
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In considering the electron microscopic study of sections of tissue as a practical approach to the study of cell structure, the cytologist is faced with a number of possible decisions with respect to the way in which he will prepare his material. Not the least of these is the problem of how thin the section of tissue should be to serve his purpose. A useful section of a sample must satisfy the ...
متن کاملHow Thin Should a Section Be?
Ornstein (1956) has recently discussed the maximum useful thickness of a section for electron microscopy, as limited by the energy loss of the beam in traversing it and by the chromatic aberration of the objective lens. For working voltages of 50 to 60 kv. his conclusion is almost identical with that which we initially established experimentally (Cosslett, 1951) and later supported by theoretic...
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ژورنال
عنوان ژورنال: Journal of Neuroscience
سال: 2008
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.2992-08.2008